Application of alkyl polyglycoside surfactant in ultrasonic-assisted extraction followed by macroporous resin enrichment for the separation of vitexin-2"-O-rhamnoside and vitexin from Crataegus pinnatifida leaves

作者：Han, Feng; Guo, Yupin; Gu, Huiyan^*; Li, Fenglan; Hu, Baozhong; Yang, Lei^*

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2016, 1012: 69-78.

DOI：10.1016/j.jchromb.2016.01.017

• Alkyl polyglycoside
• Vitexin-2"-O-rhamnoside
• Vitexin
• Ultrasonic-assisted extraction
• Macroporous resin
• Crataegus pinnatifida

An alkyl polyglycoside (APG) surfactant was used in ultrasonic -assisted extraction to effectively extract vitexin-2 ''-O-rhamnoside (VOR) and vitexin (VIT) from Crataegus pinnatifida leaves. APG0810 was selected as the surfactant. The extraction process was optimized for ultrasonic power, the APG concentration, ultrasonic time, soaking time, and liquid-solid ratio. The proposed approach showed good recovery (99.80-102.50% for VOR and 98.83-103.19% for VIT) and reproducibility (relative standard deviation, n = 5; 3.7% for VOR and 4.2% for VIT) for both components. The proposed sample preparation method is both simple and effective. The use of APG for extraction of key herbal ingredients shows great potential. Ten widely used commercial macroporous resins were evaluated in a screening study to identify a suitable resin for the separation and purification of VOR and VIT. After comparing static and dynamic adsorption and desorption processes, HPD100B was selected as the most suitable resin. After column adsorption and desorption on this resin, the target compounds VOR and VIT can be effectively separated from the APG0810 extraction solution. Recoveries of VOR and VIT were 89.27% +/- 0.42% and 85.29% +/- 0.36%, respectively. The purity of VOR increased from 35.0% to 58.3% and the purity of VIT increased from 12.5% to 19.9%.

• 出版日期2016-2-15
• 单位河北北方学院; 东北农业大学; 东北林业大学