A comparison of three rapid and accurate bioluminescent antibiotic susceptibility tests

作者：Lafond Mickael; Vidal Nicolas; Letourneux Yves; Brunel Jean Michel^*

来源：Journal of Pharmacological and Toxicological Methods, 2010, 61(1): 16-19.

DOI：10.1016/j.vascn.2009.10.004

• Bioluminescence
• Antibiotic susceptibility
• Quinone:NAD(P)H oxydoreductase

Introduction: Rapid determination of microorganisms' antibiotic susceptibility remains a great challenge for the clinical diagnosis of infectious diseases. In this study, we compare three rapid bioluminescent antibiotic susceptibility tests with the conventional normalised microdilution method. Methods: The susceptibility of two bacterial and two fungal strains to a 6 h incubation with one of eight standard antibiotics was investigated by the ATP, AK and QNO bioluminescent methods. Results: Three rapid and accurate antibiotic susceptibility testing were investigated performing ATP, AK and QNO bioluminescent assays. All the results were in accordance with those obtained using a standard microdilution assay. All these methods were sensitive and faster than the previously well-known ones. Discussion: The development of bioluminescent determination methods could lead to accurate and useful antibiotic susceptibility testing.

• 出版日期2010-2