Double-pore structure and enzyme activity of SiO2 monolithic carrier

作者：Yang Hong Bin; Chen Qi^*; Song Li; Lu Jian Ying; Li Hui Ping

来源：Chinese Journal of Inorganic Chemistry, 2007, 23(1): 164-168.

• double-pore structure
• porous monolithic
• sol-gel
• enzyme carrier
• enzyme activity

SiO2 monoliths with double-pore structure were synthesized with tetraethyl orthosilicate, polyethylene glycol, starch, ethanol and ammonia as the main raw materials via a sol-gel route. The effect of different content of starch of the samples was studied on the mesopore structure, macropore appearance, hydrothermal stability and enzyme activity to immobilize gluczyme. The results indicate that porous blocks with mesopores of 10 similar to 12 nm and macropores of 10 similar to 30 mu m can be formed by the removal of polyethylene glycol and starch after heating at 600 degrees C. The minimal density of the obtained samples was 0.34 g (.) cm(-3) and the maximal porosity was 76%. After being immersed in water at 80 T for 7 d, the density, porosity and shape of N-2 adsorption-desorption curves of the samples were almost unchanged, showing that the sample has a good hydrothermal stability. The initial enzyme activity of sample P2KD30 was 11 190 U, and definite enzyme activity could be maintained after five cycles.

• 出版日期2007-1
• 单位华东理工大学