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<jats:p> There is evidence that apoptotic cells and their components have immunmodulatory properties and signaling function. The present study investigated first whether exercise-induced apoptosis and exercise-induced mobilization of progenitor cells are similarly affected by subjects' training status and, second, whether the appearance of dying cells in the circulation might mobilize progenitor cells. CD1 SWISS mice were subjected to a 10-wk endurance training using free wheel running or served as untrained controls. Mice of both groups performed an intensive exercise test after the training period at a velocity corresponding to 80% maximal oxygen uptake for 30 min. Cells from blood and bone marrow were analyzed, and apoptosis and number of progenitor cells determined via flow cytometry. In a second experiment, apoptotic cells were transferred into recipient mice, and mobilization of progenitor cells was analyzed while vital cells served as controls. In untrained animals, the exhaustive exercise was followed by an enhanced rate of annexin V positive CD3<jats:sup>+</jats:sup> cells in blood and bone marrow ( P < 0.05), whereas no increase was found in trained mice. Similarly, exercise mobilized Sca-1<jats:sup>+</jats:sup>/c-kit<jats:sup>+</jats:sup> and Sca-1<jats:sup>+</jats:sup>/Flk<jats:sup>+</jats:sup> cells in untrained ( P < 0.05) but not trained mice. Furthermore, application of apoptotic cells and their supernatant mobilized Sca-1<jats:sup>+</jats:sup>/c-kit<jats:sup>+</jats:sup> cells into the blood ( P < 0.05), whereas Sca-1<jats:sup>+</jats:sup>/Flk<jats:sup>+</jats:sup> cells were not affected. The present study demonstrated that both lymphocyte apoptosis, as well as mobilization of progenitor cells are similarly related to training status. Furthermore, apoptotic cells seem to induce signals that effectively mobilize hematopoietic progenitor cells. The relevance of this effect for the adaptation to exercise stimuli remains to be shown. </jats:p>
- 出版日期2015-7-15
