An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology

作者：Thompson Rebecca F; Walker Matt; Siebert C Alistair; Muench Stephen P; Ranson Neil A

来源：Methods, 2016, 100: 3-15.

DOI：10.1016/j.ymeth.2016.02.017

• Electron microscopy

Transmission electron microscopy (EM) is a versatile technique that can be used to image biological specimens ranging from intact eukaryotic cells to individual proteins >150 kDa. There are several strategies for preparing samples for imaging by EM, including negative staining and cryogenic freezing. In the last few years, cryo-EM has undergone a 'resolution revolution', owing to both advances in imaging hardware, image processing software, and improvements in sample preparation, leading to growing number of researchers using cryo-EM as a research tool. However, cryo-EM is still a rapidly growing field, with unique challenges. Here, we summarise considerations for imaging of a range of specimens from macro molecular complexes to cells using EM.

• 出版日期2016-5-1