A practical fluorogenic substrate for high-throughput screening of glutathione S-transferase inhibitors

作者：Fujikawa Yuuta^*; Morisaki Fumika; Ogura Asami; Morohashi Kana; Enya Sora; Niwa Ryusuke; Goto Shinji; Kojima Hirotatsu; Okabe Takayoshi; Nagano Tetsuo; Inoue Hideshi

来源：Chemical Communications, 2015, 51(57): 11459-11462.

DOI：10.1039/c5cc02067k

We report a new fluorogenic substrate for glutathione S-transferase (GST), 3,4-DNADCF, enabling the assay with a low level of nonenzymatic background reaction. Inhibitors against Noppera-bo/GSTe14 from Drosophila melanogaster were identified by high throughput screening using 3,4-DNADCF, demonstrating the utility of this substrate.

• 出版日期2015