Competitive adsorption-desorption of IgM monomers-dimers on silica and modified silica surfaces

作者：Guha Suvajyoti; Wayment Joshua; Rastogi Vinayak; Li Mingdong; Tarlov Michael J; Zachariah Michael R^*

来源：Journal of Colloid and Interface Science, 2013, 402: 291-299.

DOI：10.1016/j.jcis.2013.02.050

• Electrospray
• Differential mobility analysis
• Protein
• Competitive adsorption
• Desorption
• Bovine
• Immunoglobulin
• IgM
• Silica surface
• Gelatin

Understanding competitive adsorption-desorption of proteins onto surfaces is an important area of research in food processing and biomedical engineering. Here, we demonstrate, how electrospray-differential mobility analysis that has been traditionally used for characterizing bionanoparticles, can be used for quantifying complex competitive adsorption-desorption of oligomeric proteins or multiprotein systems using monomers and dimers of IgM as a model example onto silica and modified silica surfaces. Using ES-DMA, we show that IgM dimers show a preference to stay adsorbed to different surfaces although monomers adsorb more easily and desorption rates of monomers and dimers of IgM are surface-type-dependent and are not significantly affected by shear. We anticipate that this demonstration will make ES-DMA a popular "label-free" method for studying multicomponent multi-oligomeric protein adsorption to different surfaces in the future. Published by Elsevier Inc.

• 出版日期2013-7-15