A brachytic dwarfism trait (dw) in peach trees is caused by a nonsense mutation within the gibberellic acid receptor PpeGID1c

作者：Hollender Courtney A; Hadiarto Toto; Srinivasan Chinnathambi; Scorza Ralph; Dardick Chris

来源：NEW PHYTOLOGIST, 2016, 210(1): 227-239.

DOI：10.1111/nph.13772

<jats:title>Summary</jats:title><jats:p>
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<jats:list-item><jats:p>Little is known about the genetic factors controlling tree size and shape. Here, we studied the genetic basis for a recessive brachytic dwarfism trait (<jats:italic>dw</jats:italic>) in peach (<jats:italic>Prunus persica</jats:italic>) that has little or no effect on fruit development.</jats:p></jats:list-item>

<jats:list-item><jats:p>A sequencing‐based mapping strategy positioned <jats:italic>dw</jats:italic> on the distal end of chromosome 6. Further sequence analysis and fine mapping identified a candidate gene for <jats:italic>dw</jats:italic> as a non‐functional allele of the gibberellic acid receptor <jats:italic>GID1c</jats:italic>.</jats:p></jats:list-item>

<jats:list-item><jats:p>Expression of the two <jats:italic>GID1‐like</jats:italic> genes found in peach, <jats:italic>PpeGID1c</jats:italic> and <jats:italic>PpeGID1b</jats:italic>, was analyzed. <jats:italic>GID1c</jats:italic> was predominantly expressed in actively growing vegetative tissues, whereas <jats:italic>GID1b</jats:italic> was more highly expressed in reproductive tissues. Silencing of <jats:italic>GID1c</jats:italic> in plum via transgenic expression of a hairpin construct led to a dwarf phenotype similar to that of <jats:italic>dw/dw</jats:italic> peaches. In general, the degree of <jats:italic>GID1c</jats:italic> silencing corresponded to the degree of dwarfing.</jats:p></jats:list-item>

<jats:list-item><jats:p>The results suggest that <jats:italic>PpeGID1c</jats:italic> serves a primary role in vegetative growth and elongation, whereas <jats:italic>GID1b</jats:italic> probably functions to regulate gibberellic acid perception in reproductive organs. Modification of <jats:italic>GID1c</jats:italic> expression could provide a rational approach to control tree size without impairing fruit development.</jats:p></jats:list-item>
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• 出版日期2016-4