Biodegradation of Isopropanol by a Solvent-Tolerant Paracoccus denitrificans Strain

作者：Geng Yucong; Deng Yuanjie; Chen Feilong; Jin Hong; Tao Ke; Hou Taiping^*

来源：Preparative Biochemistry & Biotechnology, 2015, 45(5): 491-499.

DOI：10.1080/10826068.2014.923452

• specific growth rate
• Paracoccus denitrificans
• acetone
• isopropanol (IPA)
• biodegradation
• Andrews model

The biodegradation of high concentration isopropanol (2-propanol, IPA) at 16g/L was investigated by a solvent-tolerant strain of bacteria identified as Paracoccus denitrificans for the first time by 16S rDNA gene sequencing. The strain P. denitrificans GH3 was able to utilize the high concentration of IPA as the sole carbon source within a minimal salts medium with a cell density of 1.5x10(8) cells/mL. The optimal conditions were found as follows: initial pH 7.0, incubation temperature 30 degrees C, with IPA concentration 8g/L. Under the optimal conditions, strain GH3 utilized 90.3% of IPA in 7 days. Acetone, the major intermediate of aerobic IPA biodegradation, was also monitored as an indicator of microbial IPA utilization. Both IPA and acetone were completely removed from the medium following 216hr and 240hr, respectively. The growth of strain GH3 on IPA as a sole carbon and energy source was well described by the Andrews model with a maximum growth rate (mu( max ))=0.0277/hr, a saturation constant (K ( S ))=0.7333g/L, and an inhibition concentration (Ki)=8.9887g/L. Paracoccus denitrificans GH3 is considered to be well used in degrading IPA in wastewater.

• 出版日期2015-7-4
• 单位四川大学