Monitoring of the action of drugs in melanoma cells by dynamic laser speckle

作者:Gonzalez Pena Rolando J*; Braga Roberto A Jr; Cibrian Rosa M; Salvador Palmer Rosario; Gil Benso Rosario; San Miguel Teresa
来源:Journal of Biomedical Optics, 2014, 19(5): 057008.
DOI:10.1117/1.JBO.19.5.057008

摘要

This work presents the development of a protocol based on the dynamic laser speckle designed to monitor the reaction of cancer cells of line MEL-RC08 to the application of the drug Colcemid in two different concentrations: 0.2 and 0.4 mu g/mL. The protocol was designed using the forward scattering approach with an He-Ne laser of 632.8 nm illuminating the samples, a control, and two variations of Colcemid, being monitored along 8 h. The data were analyzed numerically in the time and in the frequency domain, and the results presented the ability of the technique to monitor the action of the drug, particularly Colcemid (0.4 mu g/mL).

  • 出版日期2014-5