A maize map standard with sequenced core markers, grass genome reference points and 932 expressed sequence tagged sites (ESTs) in a 1736-locus map

作者：Davis GL; McMullen MD; Baysdorfer C; Musket T; Grant D; Staebell M; Xu G; Polacco M; Koster L; Melia Hancock S; Houchins K; Chao S; Coe EH^*

来源：Genetics, 1999, 152(3): 1137-1172.

We have constructed a 1736-locus maize genome map containing 1156 loci probed by cDNAs, 545 probed by random genomic clones, 16 by simple sequence repeats (SSRs). 14 by isozymes, and 5 by anonymous clones. Sequence information is available for 56% of the loci with 66% of the sequenced loci assigned functions. A total of 596 new ESTs were mapped from a B73 library of 5-wk-old shoots. The map contains 237 loci probed by barley, oat, wheat, rice, or tripsacum clones, which serve as grass genome reference points in comparisons between maize and other grass maps. Ninety core markers selected for low copy number, high polymorphism. and even spacing along the chromosome delineate the 100 bins on the map. The average bin size is 17 cM. Use of bill assignments enables comparison among different maize mapping populations and experiments including those involving cytogenetic stocks, mutants, or quantitative trait loci. Integration of nonmaize markers in the map extends the resources available for gene discovery beyond the boundaries of maize mapping information into the expanse of map, sequence, and phenotype information from other grass species. This map provides a foundation for numerous basic and applied investigations including studies of gene organization, gene and genome evolution, targeted cloning, and dissection of complex traits.

• 出版日期1999-7