MEG3 regulates the invasion and migration of lung cancer via targeting miR-219a and through MAPK pathway

作者：Jin, Chengyan; Li, Jindong; Shi, Donglei; Hua, Peiyan; Zhang, Guangxin; Wang, Bin^*

来源：International Journal of Clinical and Experimental Pathology, 2017, 10(6): 7144-7153.

• MEG3
• lung cancer
• MiR-219a
• transwell

Objective: To investigate the expression of MEG3 in lung cancer and the role of MEG3 in the invasion and migration of lung cancer cells and its mechanism. Methods: The expression of MEG3 in lung cancer and adjacent lung cancer cells was detected by qPCR. Transwell invasion assay was used to detect the invasion ability of lung cancer cells after silencing MEG3. The migration ability of lung cancer cells after silencing MEG3 was detected by scratch test. The expression of miR-219a was detected by flow cytometry. The expression of miR-219a in lung cancer and adjacent tissues and different lung cancer cells was detected by qPCR. Transwell invasion assay was used to detect the effect of miR-219a on the invasion of lung cancer cells after silencing MEG3. The effect of miR-219a on the tumor size and volume of lung cancer was detected by subcutaneous tumorigenesis of MEG3 in nude mice. The effect of miR-219a on the tumor size and volume of lung cancer cells was detected by scintigraphy. Western blotting was used to detect the expression of Notch pathway protein after silencing MEG3. The morphological changes of microcapsule microtubules after silencing MEG3 were detected by phalloidin staining. Results: The expression of MEG3 was significantly increased in lung cancer tissues compared with adjacent tissues; The expression of MEG3 was the highest in lung cancer cell A549; MEG3 could specifically bind to 3'UTR of miR-219a; The expression of miR-219a was significantly decreased in lung cancer tissues compared with adjacent tissues; Inhibition of miR-219a could promote the invasion and migration of lung cancer cells after silencing MEG3; The tumor volume and weight of MEG3-siRNA + miR-219a-inhibitor group were significantly increased compared with MEG3-siRNA group. The expression of Notch pathway protein was down-regulated after silencing MEG3. The number of microfilament microtubules decreased and the number of pseudopods decreased after silencing MEG3. Conclusion: MEG3 plays an important role in the development of lung cancer. MEG3 can regulate the invasion and migration ability of miR-219a through Notch signaling pathway.

• 出版日期2017
• 单位吉林大学