A facile and efficient single-step approach for the fabrication of vancomycin functionalized polymer-based monolith as chiral stationary phase for nano-liquid chromatography

作者：Xu, Dongsheng; Shao, Huikai; Luo, Rongying; Wang, Qiqin; Sanchez-Lopez, Elena; F****i, Salvatore; Luisa Marina, Maria; Jiang, Zhengjin^*

来源：Journal of Chromatography A, 2018, 1557: 43-50.

DOI：10.1016/j.chroma.2018.05.004

• Vancomycin
• Enantioseparation
• Organic polymeric monolith
• Nano-LC

A facile single-step preparation strategy for fabricating vancomycin functionalized organic polymer based monolith within 100 mu m fused-silica capillary was developed. The synthetic chiral functional monomer, i.e 2-isocyanatoethyl methacrylate (ICNEML) derivative of vancomycin, was co-polymerized with the cross-linker ethylene dimethacrylate (EDMA) in the presence of methanol and dimethyl sulfoxide as the selected porogens. The co-polymerization conditions were systematically optimized in order to obtain satisfactory column performance. Adequate permeability, stability and column morphology were observed for the optimized poly(ICNEML-vancomycin-co-EDMA) monolith. A series of chiral drugs were evaluated on the monolith in either several other beta-blockers. The proposed single-step approach not only resulted in a vancomycin functionalized organer polar organic-phase or reversed-phase modes. After the optimization of separation conditions, baseline or partial enantioseparation were obtained for series of drugs including thalidomide, colchicine, carteolol, salbutamol, clenbuterol andic polymer-based monolith with acceptable performance, but also significantly simplified the preparation procedure by reducing time and labor.

• 出版日期2018-7-6
• 单位暨南大学