Dual Selection Marker Transformation System Using Agrobacterium tumefaciens for the Industrial Aspergillus oryzae 3.042

作者：Sun, Yunlong; Niu, Yali; He, Bin; Ma, Long; Li, Ganghua; Van-Tuan Tran; Zeng, Bin^*; Hu, Zhihong^*

来源：Journal of Microbiology and Biotechnology, 2019, 29(2): 230-234.

DOI：10.4014/jmb.1811.11027

• Aspergillus oryzae
• uridine/uracil auxotrophy
• pyrithiamine
• Agrobacterium tumefaciens-mediated transformation

Currently, the genetic modification of Aspergillus oryzae is mainly dependent on protoplast-mediated transformation (PMT). In this study, we established a dual selection marker system in an industrial A. oryzae 3.042 strain by using Agrobacterium tumefaciens-mediated transformation (ATMT). We first constructed a uridine/uracil auxotrophic A. oryzae 3.042 strain and a pyrithiamine (PT)-resistance binary vector. Then, we established the ATMT system by using uridine/uracil auxotrophy and PT-resistance genes as selection markers. Finally, a dual selection marker ATMT system was developed. This study demonstrates a useful dual selection marker transformation system for genetic manipulations of A. oryzae 3.042.

• 出版日期2019-2
• 单位江西科技师范大学; 湖北师范大学