Porcine Interleukin-2 Expression in Insect Cells and Its Enhancement of Pig Immunity to Swine Influenza Virus Inactivated Vaccine

作者：Chen Hong-ying; Zhang Hong-ying; Huang Yan-quan; Cui Bao-an^*; Wang Zhen-ya; Wang Yan-bin; Liu Jin-peng; Chao An-jun

来源：Agricultural Sciences in China, 2010, 9(8): 1211-1220.

DOI：10.1016/S1671-2927(09)60209-1

• porcine interleukin-2
• Sf9 insect cells
• expression
• inactivated vaccine
• swine influenza virus

Mature porcine interleukin-2 (pIL-2) gene was amplified by PCR from the plasmid pGEM-T-pIL2 and cloned into the baculovirus pFastBac (TM) Dual vector of the Bac-to-Bac baculovirus expression system under the control of the PH promoter. Recombinant pIL-2 (rpIL-2) expressed in Sf9 insect cells was detected by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunofluorescence assay. Western blot analysis confirmed that the rpIL-2 protein had a molecular mass of 20 kDa, which was larger than the molecular mass of the mature protein predicted based on its peptide sequence. The rpIL-2 protein induced in vitro proliferation of ConA-stimulated porcine splenocytes and enhanced in vivo protective immune responses induced by vaccinating the pigs with inactivated oil emulsion vaccine against swine influenza virus. The results showed that the rpIL-2 expressed in Sf9 insect cells has immunoenhancement effects; the finding lays the foundation for the preparation of a specific recombinant IL-2 protein and the development of a novel immune adjuvant of vaccines against various infectious porcine pathogens to increase the immunoprotective efficacy of vaccines.

• 出版日期2010-8-20
• 单位河南农业大学