Melanoma cell-derived exosomes alter macrophage and dendritic cell functions in vitro

作者：Marton Annamaria; Vizler Csaba; Kusz Erzsebet; Temesfoi Viktoria; Szathmary Zsuzsa; Nagy Krisztina; Szegletes Zsolt; Varo Gyorgy; Siklos Laszlo; Katona Robert L; Tubak Vilmos; Howard O M Zack; Duda Erno; Minarovits Janos; Nagy Katalin; Buzas Krisztina^*

来源：Immunology Letters, 2012, 148(1): 34-38.

DOI：10.1016/j.imlet.2012.07.006

• Melanoma
• Tumor immunity
• Exosomes
• Cytokine profile

To clarify controversies in the literature of the field, we have purified and characterized B16F1 melanoma cell derived exosomes (mcd-exosomes) then we attempted to dissect their immunological activities. We tested how mcd-exosomes influence CD4+T cell proliferation induced by bone marrow derived dendritic cells; we quantified NF-kappa B activation in mature macrophages stimulated with mcd-exosomes, and we compared the cytokine profile of LPS-stimulated, IL-4 induced, and mcd-exosome treated macrophages. We observed that mcd-exosomes helped the maturation of dendritic cells, enhancing T cell proliferation induced by the treated dendritic cells. The exosomes also activated macrophages, as measured by NF-kappa B activation. The cytokine and chemokine profile of macrophages treated with tumor cell derived exosomes showed marked differences from those induced by either LPS or IL-4, and it suggested that exosomes may play a role in the tumor progression and metastasis formation through supporting tumor immune escape mechanisms.

• 出版日期2012-12