MRI measurement of angiogenesis and the therapeutic effect of acute marrow stromal cell administration on traumatic brain injury

作者：Li Lian; Chopp Michael; Ding Guang Liang; Qu Chang Sheng; Li Qing Jiang; Lu Mei; Wang Shiyang; Nejad Davarani Siamak P; Mahmood Asim; Jiang Quan^*

来源：Journal of Cerebral Blood Flow and Metabolism, 2012, 32(11): 2023-2032.

DOI：10.1038/jcbfm.2012.106

• angiogenesis
• marrow stromal cells
• MRI
• perfusion
• ventricular dilation

Using magnetic resonance imaging (MRI), the present study was undertaken to investigate the therapeutic effect of acute administration of human bone marrow stromal cells (hMSCs) on traumatic brain injury (TBI) and to measure the temporal profile of angiogenesis after the injury with or without cell intervention. Male Wistar rats (300 to 350 g, n=18) subjected to controlled cortical impact TBI were intravenously injected with 1 mL of saline (n=9) or hMSCs in suspension (n=9, 3x10(6) hMSCs) 6 hours after TBI. In-vivo MRI acquisitions of T2-weighted imaging, cerebral blood flow (CBF), three-dimensional (3D) gradient echo imaging, and blood-to-brain transfer constant (Ki) of contrast agent were performed on all animals 2 days after injury and weekly for 6 weeks. Sensorimotor function and spatial learning were evaluated. Volumetric changes in the trauma-induced brain lesion and the lateral ventricles were tracked and quantified using T2 maps, and hemodynamic alteration and blood-brain barrier permeability were monitored by CBF and Ki, respectively. Our data show that transplantation of hMSCs 6 hours after TBI leads to reduced cerebral atrophy, early and enhanced cerebral tissue perfusion and improved functional outcome compared with controls. The hMSC treatment increases angiogenesis in the injured brain, which may promote neurologic recovery after TBI. Journal of Cerebral Blood Flow %26 Metabolism (2012) 32, 2023-2032; doi:10.1038/jcbfm.2012.106; published online 11 July 2012

• 出版日期2012-11