A Phosphate Starvation-Inducible Ribonuclease of Bacillus licheniformis

作者：Thanh Trung Nguyen; Minh Hung Nguyen; Huy Thuan Nguyen; Hoang Anh Nguyen; Thi Hoi Le; Schweder Thomas; Juergen Britta

来源：Journal of Microbiology and Biotechnology, 2016, 26(8): 1464-1472.

DOI：10.4014/jmb.1601.01087

• Bacillus licheniformis
• barnase
• binase
• phosphate starvation
• ribonuclease

The BLi03719 protein of Bacillus licheniformis DSM13 belongs to the most abundant extracellular proteins under phosphate starvation conditions. In this study, the function of this phosphate starvation inducible protein was determined. An amino-acid sequence analysis of the BLi03719-encoding gene showed a high similarity with genes encoding the barnase of Bacillus amyloliquefaciens FZB42 and binase-like RNase of Bacillus pumilus SARF-032. The comparison of the control strain and a BLi03719-deficient strain revealed a strongly reduced extracellular ribonuclease activity of the mutant. Furthermore, this knockout mutant exhibited delayed growth with yeast RNA as an alternative phosphate and carbon source. These results suggest that BLi03719 is an extracellular ribonuclease expressed in B. licheniformis under phosphate starvation conditions. Finally, a BLi03719 mutant showed an advantageous effect on the overexpression of the heterologous amyE gene under phosphate-limited growth conditions.

• 出版日期2016-8