A multibiomarker approach to explore interactive effects of propranolol and fluoxetine in marine mussels

作者：Franzellitti Silvia^*; Buratti Sara; Du Bowen; Haddad Samuel P; Chambliss C Kevin; Brooks Bryan W; Fabbri Elena

来源：Environmental Pollution, 2015, 205: 60-69.

DOI：10.1016/j.envpol.2015.05.020

• Marine mussel
• Fluoxetine
• Propranolol
• Bioaccumulation
• Biomarkers
• Lysosomal responses
• Drug-drug interaction
• Adverse outcome pathway

A multi-biomarker approach, including several lysosomal parameters, activity and mRNA expression of antioxidant enzymes, and DNA damage, was employed to investigate the nominal effects of 0.3 ng/L fluoxetine (FX) and 0.3 ng/L propranolol (PROP) alone or in combination (0.3 ng/L FX + 0.3 ng/L PROP) on Mediterranean mussels after a 7 day treatment. FX co-exposure appears to facilitate PROP bioaccumulation because PROP only accumulated in digestive gland of FX + PROP treated mussels. Lysosomal parameters were significantly impaired by FX + PROP treatment, while no clear antioxidant responses at the catalytic and transcriptional levels were observed. Biomarker responses led to a "medium stress level" diagnosis in FX + PROP treated mussels, according to the Expert System, whereas 0.3 ng/L PROP or FX alone did not induce consistent stress conditions. These findings suggest vulnerability of coastal marine mussels to FX and PROP contamination at environmentally relevant levels.

• 出版日期2015-10