科研之友
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摘要
Objectives: Chronic administration of nicotinic acid (NA), a potent antilipidemic compound, aggravates glycemic control in diabetic patients. It is not known if NA has direct effects on islet beta cells.
Methods: Real-time reverse transcriptase-polymerase chain reaction, in situ hybridization, and immunofluorescence techniques were used to examine the expression of NA receptor PUMA-G, a member of the G protein-coupled receptor (G-PCR) family, in murine islet beta cells. Calcium transient was measured using confocal microscopy, whereas the intracellular cyclic adenosine monophosphate and glucose-stimulated insulin secretion (GSIS) from isolated islets were determined by the enzyme-linked immunosorbent assay.
Results: High levels of PUMA-G transcripts and protein were detected in all beta cells, and about 40% of alpha cells. PUMA-G transcripts increased more than 3-fold in islets incubated with interferon gamma. Cyclic adenosine monophosphate accumulation, induced by IBMX/forskolin, was inhibited by NA; however, the inhibition was completely abolished by pretreatment of the culture with pertussis toxin. No calcium transient was detected in islet cells in the presence of NA. Static incubation of islets with NA led to an approximately 30% reduction of GSIS.
Conclusions: The results indicated that PUMA-G stimulation by NA in islet beta cells inhibited GSIS likely via activation of the G(i) signaling pathway.
