An ex vivo continuous passive motion model in a porcine knee for assessing primary stability of cell-free collagen gel plugs

作者：Efe Turgay^*; Schofer Markus D; Fueglein Alexander; Timmesfeld Nina; Fuchs Winkelmann Susanne; Stein Thomas; El Zayat Bilal Farouk; Paletta Juergen R J; Heyse Thomas J

来源：BMC Musculoskeletal Disorders, 2010, 11: 283.

DOI：10.1186/1471-2474-11-283

Background: Primary stability of cartilage repair constructs is of the utmost importance in the clinical setting but few continuous passive motion (CPM) models are available. Our study aimed to establish a novel ex vivo CPM animal model and to evaluate the required motion cycles for testing the mechanical properties of a new cell-free collagen type I gel plug (CaReS (R)-1S).
Methods: A novel ex vivo CPM device was developed. Full-thickness cartilage defects (11 mm diameter by 6 mm deep) were created on the medial femoral condyle of porcine knee specimens. CaReS (R)-1S was implanted in 16 animals and each knee underwent continuous passive motion. After 0, 2000, 4000, 6000, and 8000 motions, standardized digital pictures of the grafts were taken, focusing on the worn surfaces. The percentage of worn surface on the total CaReS (R)-1S surface was evaluated with image processing software.
Results: Significant differences in the worn surface were recorded between 0 and 2000 motion cycles (p < 0.0001). After 2000 motion cycles, there was no significant difference. No total delamination of CaReS (R)-1S with an empty defect site was recorded.
Conclusion: The ex vivo CPM animal model is appropriate in investigating CaReS (R)-1S durability under continuous passive motion. 2000 motion cycles appear adequate to assess the primary stability of type I collagen gels used to repair focal chondral defects.

• 出版日期2010-12-15
• 单位河北医科大学