Detection of Staphylococcus aureus With a Fluorescence In Situ Hybridization That Does Not Require Lysostaphin

作者：Lawson Thomas S^*; Connally Russell E; Iredell Jonathan R; Vemulpad Subramanyam; Piper James A

来源：Journal of Clinical Laboratory Analysis, 2011, 25(2): 142-147.

DOI：10.1002/jcla.20448

• early diagnosis
• fluorescent in situ hybridization
• gram-positive bacteria
• molecular diagnostic
• Staphylococcus aureus
• lysostaphin
• lysozyme
• techniques

To detect with whole-cell fluorescence in situ hybridization (FISH), Staphylococcus aureus is typically permeabilized with lyozyme and lysostaphin. We tested whether it was feasible to detect S. aureus and differentiate it from Staphylococcus epidermidis with lysozyme-only permeabilization. We compared lysozyme permeabilization to S. aureus permeabilized with lysozyme in combination with lysostaphin. It was determined that S. aureus treated with agarose, methanol, and lysozyme could be detected with FISH. The 1 hr protocol is a useful alternative to conventional FISH. J. Clin. Lab. Anal. 25:142-147, 2011.

• 出版日期2011