A Series of Novel Rare Earth Molybdotungstosilicate Heteropolyoxometalates Binding to Bovine Serum Albumin: Spectroscopic Approach

作者：Hu, Yan-Jun; Yu Ou-Yang; Bai, Ai-Min; Zhao, Ru-Ming; Liu, Yi^*

来源：Biological Trace Element Research, 2010, 136(1): 8-17.

DOI：10.1007/s12011-009-8521-8

• Rare earth
• Molybdotungstosilicate heteropolyoxometalates
• Bovine serum albumin
• Thermodynamic parameters
• Spectroscopic approach

Heteropolyoxometalate complexes have been widely applied in many fields. In this paper, the interaction between a series of novel rare earth molybdotungstosilicate heteropolyoxometalates, K(10)H(3)[Ln(SiMo(6)W(5)O(39))(2)]center dot xH(2)O (abbr. LnW(5), Ln = Pr (x = 30), Gd (x = 29), Dy (x = 28), and Yb (x = 31)), and bovine serum albumin (BSA) was investigated by spectroscopic approach under the physiological conditions. In the mechanism discussion, it was proved that the fluorescence quenching of BSA by LnW(5) is a result of the formation of LnW(5)-BSA complex. Fluorescence quenching constants were determined using the Stern-Volmer equation to provide a measure of the binding affinity between LnW(5) and BSA. The binding affinity ranked in the order GdW(5) > DyW(5) > PrW(5) > YbW(5). The results of thermodynamic parameters Delta G, Delta H, and Delta S at different temperatures indicate that van der Waals interactions and hydrogen bonds play a major role for LnW(5)-BSA association. Furthermore, the distance r between donor (BSA) and acceptor (LnW(5)) was obtained according to fluorescence resonance energy transfer.

• 出版日期2010-7
• 单位武汉大学; 病毒学国家重点实验室; 湖北师范大学