Methylation specific targeting of a chromatin remodeling complex from sponges to humans

作者：Cramer Jason M; Pohlmann Deborah; Gomez Fernando; Mark Leslie; Kornegay Benjamin; Hall Chelsea; Siraliev Perez Edhriz; Walavalkar Ninad M; Sperlazza M Jeannette; Bilinovich Stephanie; Prokop Jeremy W; Hill April L; Williams David C Jr^*

来源：Scientific Reports, 2017, 7(1): 40674.

DOI：10.1038/srep40674

DNA cytosine methylation and methyl-cytosine binding domain (MBD) containing proteins are found throughout all vertebrate species studied to date. However, both the presence of DNA methylation and pattern of methylation varies among invertebrate species. Invertebrates generally have only a single MBD protein, MBD2/3, that does not always contain appropriate residues for selectively binding methylated DNA. Therefore, we sought to determine whether sponges, one of the most ancient extant metazoan lineages, possess an MBD2/3 capable of recognizing methylated DNA and recruiting the associated nucleosome remodeling and deacetylase (NuRD) complex. We find that Ephydatia muelleri has genes for each of the NuRD core components including an EmMBD2/3 that selectively binds methylated DNA. NMR analyses reveal a remarkably conserved binding mode, showing almost identical chemical shift changes between binding to methylated and unmethylated CpG dinucleotides. In addition, we find that EmMBD2/3 and EmGATAD2A/B proteins form a coiled-coil interaction known to be critical for the formation of NuRD. Finally, we show that knockdown of EmMBD2/3 expression disrupts normal cellular architecture and development of E. muelleri. These data support a model in which the MBD2/3 methylation-dependent functional role emerged with the earliest multicellular organisms and has been maintained to varying degrees across animal evolution.

• 出版日期2017-1-17
• 单位MIT