Active Conformation of DNA Aptamer Against Recombinant Human Erythropoietin-alpha

作者：He Xiaoqin; He Junlin; Xu Hua; Guo Lei^*; Xie Jianwei

来源：Chemical Journal of Chinese Universities, 2018, 39(1): 48-53.

DOI：10.7503/cjcu20170312

• Surface plasmon resonance
• Circular dichroism
• Fluorescent probes
• G-Quadruplex
• Sodium cation

A series of spectroscopic techniques such as surface plasmon resonance, circular dichroism and fluorescence was employed to illustrate the relationship between the binding activity and conformation of the shortest aptamer In27 against recombinant human erythropoietin-alpha(EPO-alpha). It was found that the presence and concentration of sodium cation were the key factors that affected the specific affinity recognition of aptamer In27. Sodium cation induced aptamer In27 to form a parallel/antiparallel hybrid G-quadruplex(GQ) conformation. GQ-specific fluorescent probe thysmenonine T(ThT) specifically bound to the hybrid GQ conformation of aptamer In27. The addition of EPO-alpha could compete with ThT to facilitate to create a label-free fluorescent "turn-off" probe for EPO-alpha. This research provides an effective combination of techniques for elucidating the active conformation of novel aptamers, and provides a kind of aptameric modules with clear interaction features for analytical, sensing and diagnostic applications.

• 出版日期2018-1-10
• 单位中国人民解放军军事医学科学院