Aqueous normal-phase chromatography using silica-hydride-based stationary phases

作者：Pesek Joseph J^*; Matyska Maria T; Boysen Reinhard I; Yang Yuanzhong; Hearn Milton T W

来源：TrAC-Trends in Analytical Chemistry, 2013, 42: 64-73.

DOI：10.1016/j.trac.2012.09.016

• High-performance liquid chromatography (HPLC)
• Hydrophilic retention
• Metabolite
• Natural product
• Normal-phase chromatography
• Peptide
• Pharmaceutical
• Separation
• Silica hydride
• Stationary phase

Aqueous normal-phase chromatography utilizing silica-hydride-based stationary phases provides unique selectivities for the separation of polar and non-polar compounds and offers new avenues to solve difficult analytical separations. By varying the amount of water in the mobile phase, all silica-hydride-based separation materials can operate in the reversed-phase mode, the normal-phase mode or, in some instances, a combination of both. %26lt;br%26gt;This review describes some of the fundamental properties of silica-hydride materials and applications that encompass a broad range of separation challenges. Moreover, exemplars are provided that illustrate many of the prominent features that lead to the selection of such stationary phases for solving a particular analytical problem.

• 出版日期2013-1