A Kaposi%26apos;s Sarcoma-Associated Herpesvirus MicroRNA and Its Variants Target the Transforming Growth Factor beta Pathway To Promote Cell Survival

作者：Lei Xiufen; Zhu Ying; Jones Tiffany; Bai Zhiqiang; Huang Yufei; Gao Shou Jiang^*

来源：Journal of Virology, 2012, 86(21): 11698-11711.

DOI：10.1128/JVI.06855-11

Transforming growth factor beta (TGF-beta) signaling regulates cell growth and survival. Dysregulation of the TGF-beta pathway is common in viral infection and cancer. Latent infection by Kaposi%26apos;s sarcoma-associated herpesvirus (KSHV) is required for the development of several AIDS-related malignancies, including Kaposi%26apos;s sarcoma and primary effusion lymphoma (PEL). KSHV encodes more than two dozen microRNAs (miRs) derived from 12 pre-miRs with largely unknown functions. In this study, we show that miR variants processed from pre-miR-K10 are expressed in KSHV-infected PEL cells and endothelial cells, while cellular miR-142-3p and its variant miR-142-3p_-1_5, which share the same seed sequence with miR-K10a_+1_5, are expressed only in PEL cells and not in uninfected and KSHV-infected TIME cells. KSHV miR-K10 variants inhibit TGF-beta signaling by targeting TGF-beta type II receptor (T beta RII). Computational and reporter mutagenesis analyses identified three functional target sites in the T beta RII 3%26apos; untranslated region (3%26apos;UTR). Expression of miR-K10 variants is sufficient to inhibit TGF-beta-induced cell apoptosis. A suppressor of the miRs sensitizes latent KSHV-infected PEL cells to TGF-beta and induces apoptosis. These results indicate that miR-K10 variants manipulate the TGF-beta pathway to confer cells with resistance to the growth-inhibitory effect of TGF-beta. Thus, KSHV miRs might target the tumor-suppressive TGF-beta pathway to promote viral latency and contribute to malignant cellular transformation.

• 出版日期2012-11