Adiponectin corrects premature cellular senescence and normalizes antimicrobial peptide levels in senescent keratinocytes

作者：Jin Taewon; Kim Min Jeong; Heo Won Il; Park Kui Young; Choi Sun Young; Lee Mi Kyung; Hong Seung Phil; Kim Seong Jin; Im Myung; Moon Nam Ju; Seo Seong Jun^*

来源：Biochemical and Biophysical Research Communications, 2016, 477(4): 678-684.

DOI：10.1016/j.bbrc.2016.06.119

• Adiponectin
• Keratinocyte
• Senescence
• Antimicrobial peptide
• SIRT1

Stress-induced premature senescence or aging causes dysfunction in the human somatic system. Adiponectin (Acrp30) plays a role in functional recovery, especially with adenosine 3',5'-monophosphate (AMP)-activated protein kinase (AMPK) and silent mating type information regulation 2 homolog 1 (SIRT1). Acrp30 stimulation reduced the premature senescence positive ratio induced by hydrogen peroxide (H2O2) and restituted human beta-defensin 2 (hBD-2) levels in senescent keratinocytes. Acrp30 recovered AMPK activity in senescent keratinocytes and increased SIRT1 deacetylation activity. As a result, FoxO1 and FoxO3 transcription activity was recovered. Additionally, Acrp30 stimulation suppresses NF kappa B p65, which induces abnormal expression of hBD-2 induced by H2O2. In the present study, we have shown that Acrp30 reduces premature senescence and recovers cellular function in keratinocytes. These results suggest a role for Acrp30 as an anti-aging agent to improve impaired skin immune barriers.

• 出版日期2016-9-2