Development and validation of an inexpensive and efficient method for the extraction of Theileria orientalis DNA from blood

作者：Bogema D R; Fell S A; O'Rourke B A; Collins D; Eamens G J; Jenkins C^*

来源：Veterinary Parasitology, 2015, 212(3-4): 379-381.

DOI：10.1016/j.vetpar.2015.08.014

• Theileria orientalis
• DNA extraction
• DPK method
• qPCR
• Genotypes

Theileria orientalis is an emerging bovine pathogen in Australasia. PCR-based detection methods for this parasite are sensitive but relatively expensive, partly due to costs associated with DNA extraction. An inexpensive and efficient technique was developed for the extraction of T. orientalis DNA from blood based on hypotonic erythrocyte lysis and detergent-proteinase K treatment (DPK method). The DPK method compares favourably to a commercial extraction kit when paired with a T. orientalis multiplex qPCR.

• 出版日期2015-9-15