Potential of RAPD analysis of the promiscuous nodulation trait in soybean (Glycine max L)

作者：Gwata Eastonce T^*; Wofford David S

来源：Biology and Fertility of Soils, 2013, 49(2): 241-244.

DOI：10.1007/s00374-012-0743-9

• Decamer
• RAPD fragment
• Promiscuous nodulation
• Rhizobia

Inoculation of soybean with cowpea-type rhizobia results in either a promiscuous type of nodulation which produces a plant with functional nodules capable of N fixation and has green leaves without N fertilization or nonpromiscuous type of nodulation which forms nonfunctional (or no nodules) and develops yellow leaves without N fertilization. Promiscuous soybean types are desirable in production regions where the availability of commercial inoculants is limited. Plant breeding efforts to develop improved promiscuous cultivars particularly in developing countries require inexpensive molecular tools for laboratory-based germplasm selection in order to reduce lengthy conventional breeding cycles. The objective of this study was to evaluate the potential of random amplified polymorphic DNA (RAPD) method in identifying promiscuous soybean genotypes. Segregating plant populations created by reciprocal crosses of promiscuous x nonpromiscuous soybean lines were evaluated for useful RAPD markers for promiscuous nodulation. One hundred and sixty random decamers of arbitrary sequences were used in screening for polymorphic loci between the two parental lines. A RAPD pattern which is consistent with the soybean genotypes segregating for promiscuous nodulation was generated by one decamer, OPB06 (5'-TGCTCTGCCC-3'), indicating the potential of using RAPD markers in selecting for promiscuity in soybean breeding programs.

• 出版日期2013-2