A real-time polymerase chain reaction method for the identification of four commercially important salmon and trout species

作者：Feng, Junli; Wu, Zhigang; Xie, Xiao; Dai, Zhiyuan^*; Liu, Shasha

来源：Mitochondrial DNA Part A, 2017, 28(1-2): 104-111.

DOI：10.3109/19401736.2015.1111346

• Control region
• quantitative real-time PCR
• salmon
• species identification

A duplex quantitative real-time PCR (qPCR) assay was developed for rapid and accurate identification of four commercially important salmon and trout species (Oncorhynchus keta, Oncorhynchus nerka, Oncorhynchus mykiss, and Salmo solar) commonly used for production process of fish in China. The assays targeting the mitochondria! control region (CR) and 16S rRNA gene were able to simultaneously discriminate four target species and the family Salmonidae from processed as well as fresh fish. The qPCR efficiency of each reaction was calculated according to the standard curve, and the method was validated by amplification DNA extracted from single or artificial mixtures prepared with the reference salmon and trout species. Testing of 11 commercial salmon and trout products by the established qPCR assay demonstrated that it was really a useful and academic technique to identify four commercially important salmon and trout species.

• 出版日期2017-3
• 单位浙江工商大学; 浙江理工大学