A simple and rapid colorimetric probe for uric acid detection based on redox reaction of 3,3 ',5,5 '-tetramethylbenzidine with HAuCl4

作者：Wang, Jing; Fang, Xian; Zhang, Yuhua; Cui, Xiaoqing; Zhao, Hong^*; Li, Xiangjun^*; Li, Zengxi^*

来源：Colloids and Surfaces A: Physicochemical and Engineering Aspects , 2018, 555: 565-571.

DOI：10.1016/j.colsurfa.2018.07.040

• 3,3 ',5,5 '-tetramethylbenzidine
• Hydrogen tetrachloroaurate hydrate
• Uric acid
• Colorimetric assay

A simple and economical colorimetric probe for the detection of uric acid (UA) based on redox reaction of 3,3',5,5'-tetramethylbenzidine (TMB) with HAuCl4 was designed. A novel chromogenic reaction was primarily demonstrated based on the reaction between HAuCl4 and TMB, which produces many color responses from red to blue in the resulting solutions. In this method, the colorless TMB can be oxidized by HAuCl4 to generate oxidized TMB (oxTMB) and induce a blue color solution which corresponding to the absorption peak at 652 nm. However, HAuCl4 would be reduced in the presence of UA, which inducing a blue color diminishing of HAuCl4 TMB system and decrease of the absorbance at 652 nm because the content of HAuCl4 was declined in the solution. Based on this phenomenon, we proposed a method to detect UA which does not require nanomaterials or organic probes. The detection conditions, pH value, incubation time and the concentration of HAuCl4 and TMB were optimized. The probe displayed a linear response in the range of 0.001-8.0 mu M and 8.0-80.0 mu M, with a detection limit of 0.67 nM (S/N = 3). This method has been successfully applied to the determination of uric acid in human urine samples.

• 出版日期2018-10-20
• 单位中国科学院大学