A simple method for the determination of reduction potentials in heme proteins

作者：Efimov Igor; Parkin Gary; Millett Elizabeth S; Glenday Jennifer; Chan Cheuk K; Weedon Holly; Randhawa Harpreet; Basran Jaswir; Raven Emma L^*

来源：FEBS LETTERS, 2014, 588(5): 701-704.

DOI：10.1016/j.febslet.2013.12.030

• Heme
• Heme protein
• Reduction potential
• Redox

We describe a simple method for the determination of heme protein reduction potentials. We use the method to determine the reduction potentials for the PAS-A domains of the regulatory heme proteins human NPAS2 (E-m = 115 mV +/- 2 mV, pH 7.0) and human CLOCK (E-m = 111 mV +/- 2 mV, pH 7.0). We suggest that the method can be easily and routinely applied to the determination of reduction potentials across the family of heme proteins.

• 出版日期2014-3-3