Antibacterial activity of silver: The role of hydrodynamic particle size at nanoscale

作者：Khurana Chandni; Vala Anjana K; Andhariya Nidhi; Pandey O P; Chudasama Bhupendra^*

来源：Journal of Biomedical Materials Research Part A, 2014, 102(10): 3361-3368.

DOI：10.1002/jbm.a.35005

• silver nanoparticles
• antibacterial activity
• microorganism
• hydrodynamic size
• minimum inhibitory concentration

Silver shows the highest antimicrobial activities amongst all metals. It is better than many first line antibiotics. The antimicrobial properties of silver can be tuned by altering its physical and surface properties. Researchers have demonstrated enhancement in the antibacterial properties of silver with decreasing particle size from bulk to nano. In the present article, we study the effect of particle size of silver at nanoscale on their antimicrobial properties. Two samples of silver nanoparticles (SNPs) of same physical size (approximate to 8 nm) but different hydrodynamic size (59 and 83 nm) are prepared by chemical reduction of AgNO3 with oleylamine followed by phase transfer with triblock copolymer Pluronic F-127. Their antimicrobial properties are investigated by microdilution method against clinically important strains of gram positive (S. aureus and B. megaterium) and gram negative (P. vulgaris and S. sonnei) bacteria. Nearly 38-50% enhancement in the antibacterial action of SNPs was observed when their hydrodynamic size was reduced to 59 nm from 83 nm. It has been observed that the antibacterial action of SNPs was governed by their hydrodynamic size and not by their crystallite and physical size. The phenomenological model was also proposed which makes an attempt to explain the microscopic mechanism responsible for the size dependent antibacterial activities of silver.

• 出版日期2014-10