Application of molecular, microbiological, and immunological tests for the diagnosis of bone and joint tuberculosis

作者：Tang, Yinghua; Yin, Lianli^*; Tang, Shifu; Zhang, Hongyu; Lan, Jihui

来源：Journal of Clinical Laboratory Analysis, 2018, 32(2): e22260.

DOI：10.1002/jcla.22260

• bone and joint tuberculosis
• TGF-
• TNF-
• Xpert
• -interferon

BackgroundTo evaluate the application of interferon gamma release assay (IGRA), rifampicin resistant real-time fluorescence quantitative PCR technique Xpert Mycobacterium tuberculosis/rifampicin (Xpert MTB/RIF), and the levels of TNF- and TGF- in the diagnosis of bone and joint tuberculosis.
MethodsEighty-six patients with bone and joint tuberculosis, diagnosed by pathology or microbiology, were examined by Xpert MTB/RIF and IGRA (T-SPOT. TB) for Mycobacterium tuberculosis infection, and the TNF- and TGF- levels of the patients were measured.
ResultsThe sensitivity of IGRA in diagnosing bone and joint tuberculosis was 81.4%; Xpert MTB/RIF's sensitivity was 70.9%. The combined sensitivity of the two methods was 91.9%. The combined detection sensitivity of the two methods was higher than individual IGRA or Xpert MTB/RIF detection sensitivity. The TNF- and TGF- levels in bone and joint tuberculosis patients were higher than those in the control group.
ConclusionXpert MTB/RIF, IGRA, TNF-, and TGF-s expression have value in the rapid diagnosis of bone and joint tuberculosis, and the sensitivity and accuracy of bone and joint tuberculosis diagnosis by combining them can improve it.

• 出版日期2018-2
• 单位广西壮族自治区人民医院; 广西中医药大学; 柳州市中医院