DIFFERENCES BETWEEN, AND POSSIBLE ORIGINS OF, THE CYTOPLASMS FOUND IN FERTILE AND MALE-STERILE ONIONS (ALLIUM-CEPA L)

作者：HOLFORD P; CROFT JH; NEWBURY HJ

来源：Theoretical and Applied Genetics, 1991, 82(6): 737-744.

DOI：10.1007/bf00227319

• ALLIUM-CEPA
• CHLOROPLASTAL DNA
• CYTOPLASMIC MALE STERILITY
• MITOCHONDRIAL DNA
• RESTRICTION FRAGMENT LENGTH POLYMORPHISM

The DNA of the organellar genomes of Allium cepa has been examined to detect restriction fragment length polymorphisms. Differences can be shown between both the chloroplastal and mitochondrial genomes of the N and cms-S cytoplasms in their restriction fragment profiles. Southern blot analysis of the mtDNA profiles using probes containing defined mitochondrial genes also detected polymorphisms. No differences can be shown between the organellar genomes of the N and cms-T onions by either of these techniques. These data indicate different origins for the two sterility-conferring cytoplasms, suggesting autoplasmic and alloplasmic origins for the cms-T and cms-S cytoplasms, respectively. No evidence of the presence of virus-like particles was found in any of the cytoplasms.

• 出版日期1991