Amperometric immunosensor for diagnosis of BLV infection

作者：Kurtinaitiene Bogumila; Ambrozaite Dovile; Laurinavicius VaIdas; Ramanaviciene Almira; Ramanavicius Arunas^*

来源：Biosensors and Bioelectronics, 2008, 23(10): 1547-1554.

DOI：10.1016/j.bios.2008.01.014

• immunosensor
• NanoBioTechnology
• HRP
• redox mediator
• electrochemical sensor
• TMB
• FCA
• BLV

A new amperometric immunosensor for detection of antibodies against bovine leukemia protein (gp51) was designed. The detection of antibody-antigen complex formation was based on application of secondary antibodies labeled with horseradish peroxidase (HRP). Ferrocenecarboxylic acid (FCA) and N,N,N',N'-tetramethylbenzidine (TMB) were selected as suitable mediators for this immunosensor. Optimal conditions for amperometric detection were found. Sensitivity of created system was compared with the results of enzyme-linked immunosorbent assay (ELISA) and agar gel immunodiffusion (AGID) reaction, and was sufficient for detection of usual anti-gp51 antibody concentration present in the blood serum of BLV-infected cattle.

• 出版日期2008-5-15