A rhodamine chromene-based turn-on fluorescence probe for selectively imaging Cu2+ in living cell

作者：Liu Wei Yong; Li Hai Ying; Lv Hong Shui; Zhao Bao Xiang^*; Miao Jun Ying

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2012, 95: 658-663.

DOI：10.1016/j.saa.2012.04.073

• Rhodamine chromene
• X-ray
• Fluorescence probe
• Copper ion
• Living cells

We describe the development of a rhodamine chromene-based turn-on fluorescence probe to monitor the intracellular Cu2+ level in living cells. The new fluorescent probe with a chlorine group in chromene moiety exhibits good membrane-permeable property than previous reported because the predicted lipophilicity of present probe 4 is stronger than that of methoxyl substituted probe in our previous work (CLogP of 4: 8.313, CLogP of methoxyl substituted probe: 7.706), and a fluorescence response toward Cu2+ under physiological conditions with high sensitivity and selectivity, and facilitates naked-eye detection of Cu2+. The fluorescence intensity was remarkably increased upon the addition of Cu2+ within 1 or 2 min, while the other sixteen metal ions caused no significant effect.

• 出版日期2012-9
• 单位山东大学