Activation of EphB2 Forward Signaling Enhances Memory Consolidation

作者：Alapin Jessica M; Dines Monica; Vassiliev Maria; Tamir Tal; Ram Alon; Locke Clifford; Yu Ji; Lamprecht Raphael^*

来源：Cell Reports, 2018, 23(7): 2014-2025.

DOI：10.1016/j.celrep.2018.04.042

EphB2 is involved in enhancing synaptic transmission and gene expression. To explore the roles of EphB2 in memory formation and enhancement, we used a photoactivatable EphB2 (optoEphB2) to activate EphB2 forward signaling in pyramidal neurons in lateral amygdala (LA). Photoactivation of optoEphB2 during fear conditioning, but not minutes afterward, enhanced long-term, but not short-term, auditory fear conditioning. Photoactivation of optoEphB2 during fear conditioning led to activation of the cAMP/Ca2+ responsive element binding (CREB) protein. Application of light to a kinase-dead optoEphB2 in LA did not lead to enhancement of long-term fear conditioning memory or to activation of CREB. Long-term, but not short-term, auditory fear conditioning memory was impaired in mice lacking EphB2 forward signaling (EphB2(lacZ/lacZ)). Activation of optoEphB2 in LA of EphB2(lacZ/lacZ) mice enhanced long-term fear conditioning memory. The present findings show that the level of EphB2 forward signaling activity during learning determines the strength of long-term memory consolidation.

• 出版日期2018-5-15