New Bacterial Compositions in Root-filled Teeth with Periradicular Lesions

作者：Schirrmeister Joerg F^*; Liebenow Anna Lisa; Pelz Klaus; Wittmer Annette; serr Annerose; Hellwig Elmar; Al Ahmad Ali

来源：Journal of Endodontics, 2009, 35(2): 169-174.

DOI：10.1016/j.joen.2008.10.024

• 16S rRNA
• endodontic microbiology
• endodontic retreatment
• molecular biology
• Vagococcus fluvialis

The aim of this study was to isolate and detect microorganisms of root-filled teeth associated with periradicular lesions. Specimens were sampled from patients undergoing root canal retreatment. The bacteria were characterized by morphologic and biochemical analysis and by 16S rRNA gene sequencing. Microorganisms were detected in 10 of 18 teeth. The majority of positive samples revealed a mixed culture of 2-8 species. In 2 teeth Enterococcus faecalis was the only detected species. For the first time Vagococcus fluvialis was detected in root canals. Solobacterium moorei and Fusobacterium nucleatum were the most prevalent species. Presence of F. nucleatum, was associated with the presence of S. moorei in 5 of 7 cases. In all teeth with Parvimonas micra and Dialister invisus, F. nucleatum and S. moorei were found. Moreover, members of additional different genera were detected delivering bacterial compositions that have been not described yet. (J Endod 2009;35:169-174)

• 出版日期2009-2
• 单位河北医科大学