Determination of florfenicol residues in broiler meat and liver samples using RP-HPLC with UV-visible detection

作者：Nasim Asma; Aslam Bilal; Javed Ijaz; Ali Asghar; Muhammad Faqir; Raza Ahmad; Sindhu Zia ud Din

来源：Journal of the Science of Food and Agriculture, 2016, 96(4): 1284-1288.

DOI：10.1002/jsfa.7220

<jats:title>Abstract</jats:title><jats:sec><jats:title>BACKGROUND</jats:title><jats:p>Broilers are vulnerable to various types of microorganisms, including <jats:italic>Salmonella</jats:italic>, <jats:italic>Escherichia coli</jats:italic> and <jats:italic>Staphylococcus aureus</jats:italic>, resulting in multiple infections. Broad‐spectrum antibacterial drugs such as florfenicol (<jats:styled-content style="fixed-case">FF</jats:styled-content>) are widely used in the treatment of such infections. Suspected residues of these drugs in body tissues of treated birds can be passed to humans through meat consumption and thus lead to serious ill effects on human health. The present study was designed to estimate the presence of <jats:styled-content style="fixed-case">FF</jats:styled-content> residues in broiler meat and liver samples.</jats:p></jats:sec><jats:sec><jats:title>RESULTS</jats:title><jats:p>The mean residual concentrations of <jats:styled-content style="fixed-case">FF</jats:styled-content> in broiler meat and liver samples were 311.42 ± 186.56 and 2585.44 ± 1759.71 µg kg<jats:sup>−1</jats:sup> respectively, which are higher than their respective maximum residual limits (<jats:styled-content style="fixed-case">MRLs</jats:styled-content>). The results showed that 126 and 24 samples were <jats:styled-content style="fixed-case">FF</jats:styled-content>‐positive and <jats:styled-content style="fixed-case">FF</jats:styled-content>‐negative respectively. Of the positive samples, 84 and 42 samples were above and below the <jats:styled-content style="fixed-case">MRL</jats:styled-content> respectively.</jats:p></jats:sec><jats:sec><jats:title>CONCLUSION</jats:title><jats:p>The results indicate the presence of <jats:styled-content style="fixed-case">FF</jats:styled-content> residues in broiler meat and liver samples. Usage of this contaminated meat causes resistance in consumers and poses a public health threat. Thus there is a need to educate farmers about the ill effects of residual drugs on human health and their withdrawal times in poultry.

• 出版日期2016-3-15