Airway Epithelial Repair by a Prebiotic Mannan Derived from Saccharomyces cerevisiae

作者：Michael Christie F; Waters Christopher M; LeMessurier Kim S; Samarasinghe Amali E; Song Chi Y; Malik Kafait U; Lew D Betty

来源：Journal of Immunology Research, 2017, 2017: 8903982.

DOI：10.1155/2017/8903982

<jats:p>In asthmatic airways, repeated epithelial damage and repair occur. No current therapy directly targets this process. We aimed to determine the effects of mannan derived from <jats:italic>S. cerevisiae</jats:italic> (SC-MN) on airway epithelial wound repair, in vitro<jats:italic>.</jats:italic> The presence of functional mannose receptors in bronchial epithelial cells was shown by endocytosis of colloidal gold-Man BSA via clathrin-coated pits in 16HBE cells. In primary normal human bronchial epithelial cells (NHBEC), SC-MN significantly facilitated wound closure. Treatment with SC-MN stimulated cell spreading as indicated by a significant increase in the average lamellipodial width of wound edge 16HBE cells. In addition, NHBEC treated with SC-MN showed increased expression and activation of Krüppel-like factors (KLFs) 4 and 5, transcription factors important in epithelial cell survival and regulation of epithelial-mesenchymal transition. We conclude that SC-MN facilitates wound repair in human bronchial epithelium, involving mannose receptors.</jats:p>

• 出版日期2017
• 单位University of Tennessee Health Science Center; The University of Tennessee Health Science Center