Hybrid molecularly imprinted polymers synthesized with 3-aminopropyltriethoxysilane-methacrylic acid monomer for miniaturized solid-phase extraction: A new and economical sample preparation strategy for determination of acyclovir in urine

作者：Yan Hongyuan^*; Wang Mingyu; Han Yehong; Qiao Fengxia; Row Kyung Ho

来源：Journal of Chromatography A, 2014, 1346: 16-24.

DOI：10.1016/j.chroma.2014.04.045

• Hybrid molecularly imprinted polymers
• 3-Aminopropyltriethoxysilane-methacrylic
• acid monomer
• Miniaturized molecularly imprinted
• solid-phase extraction
• Acyclovir
• Urine

The miniaturized molecularly imprinted solid-phase extraction (mini-MISPE) coupled with high-performance liquid chromatography was proposed for the determination of acyclovir in urine. 1.5-mL tapered plastic centrifuge tube filled with hybrid molecularly imprinted polymers (HMIPs) was used as the cartridge of mini-MISPE, and the HMIPs synthesized with 3-aminopropyltriethoxy silane-methacrylic acid as monomer exhibited good recognition and selectivity for acyclovir. Under the optimized condition, good linear calibration was obtained in a range of 0.5-15 mu g mL(-1) with the correlation coefficient of 0.9994, and the recoveries at three spiked levels were 91.6-103.3% in urine with the relative standard deviation (RSD) of %26lt;= 3.5%. Excellent intra-day and inter-day repeatability were achieved with RSD of %26lt;= 2.6% and 4.0% in three different concentrations. This method combined the advantages of HMIPs and mini-MISPE, and it could become an alternative tool for analyzing the residues of acyclovir in complex urine matrices.

• 出版日期2014-6-13
• 单位河北大学; 保定学院