NMR spectroscopic structural characterization of a water-soluble beta-(1 -> 3, 1 -> 6)-glucan from Aureobasidium pullulans

作者：Kono Hiroyuki^*; Kondo Nobuhiro; Hirabayashi Katsuki; Ogata Makoto; Totani Kazuhide; Ikematsu Shinya; Osada Mitsumasa

来源：Carbohydrate Polymers, 2017, 174: 876-886.

DOI：10.1016/j.carbpol.2017.07.018

• beta-(1 -> 3, 1 -> 6)-Glucann
• NMR
• Schizophyllan
• Aureobasidium pullulans
• Polysaccharide-polynucleotide complex

An unambiguous structural characterization of the water-soluble Aureobasidium pullulans beta-(1 -> 3, 1 -> 6)-glucan is yet to be achieved, although this beta-(1 -> 3,1 -> 6)-glucan is expected to exhibit excellent biofunctional properties. Thus, we herein report the elucidation of the primary structure of the A. pullulans beta-(1 -> 3,1 -> 6)-glucan using nuclear magnetic resonance spectroscopy, followed by comparison of the obtained structure with that of schizophyllan (SPG). Structural characterization of the A. pullulans beta-(1 -> 3,1 -> 6)-glucan revealed that the structural units are a beta-(1 -> 3,)-D-glucan backbone with four beta-(1 -> 6)-D-glucosyl side branching units every six residues. In addition, circular dichroism spectroscopic analysis revealed that the beta-(1 -> 3,1 -> 6)-glucan interacted with polyadenylic acid (poly(A)) chains in DMSO solution to form a complex similar to that obtained in the complexation of SPG/poly(A). This finding indicates that beta-(1 > 3,1 > 6)-glucan forms a triple-helical conformation in aqueous solution but exhibits a random coil structure in DMSO solution, which is similar to the behavior of SPG.

• 出版日期2017-10-15