New investigations into the stability of Mesna using LC-MS/MS and NMR

作者：Salman Dahlia^*; Swinden Julian; Peron Jean Marie R; Barton Stephen; Nabhani Gebara Shereen

来源：Expert Review of Anticancer Therapy, 2016, 16(1): 123-130.

DOI：10.1586/14737140.2016.1121106

• Mesna
• Dimesna
• stability
• chemotherapy
• sarcoma

Introduction: It is important for sarcoma patients to receive the correct dose of Mesna as an adjuvant with ifosfamide to reduce the risk of hemorrhagic cystitis. This paper describes a study conducted to evaluate the physicochemical stability of Mesna for injection formulation over 14 days. Methods: Mesna samples (n=4, 20mg/ml) were incubated in glass vials at 37+0.5oC. Mesna concentrations were determined by liquid chromatography-mass spectrometry (LC-MS/MS), and nuclear magnetic resonance spectroscopy (NMR) was used to detect degradation products. Evaporative losses and pH were also monitored. Results: Our results differed from those published in existing literature. Both LC-MS/MS and NMR indicated that Mesna was unstable. The mean percentage decrease in Mesna concentration was 40% by day 14 of the analysis. The presence of Mesna's dimer Dimesna was detected on day 0 and its concentration increased over time. Dimesna was the only by-product identified. Conclusion: Both LC-MS/MS and NMR analyses confirmed the instability of Mesna and its conversion into Dimesna.

• 出版日期2016-1-2