Next-generation Sequencing for microRNA Profiling: MicroRNA-21-3p Promotes Oral Cancer Metastasis

作者：Tseng, Hui Hwa; Tseng, Yu Kai; You, Jyun Jie; Kang, Bor Hwang; Wang, Tsung Han; Yang, Cheng Mei; Chen, Hung Chih; Liou, Huei Han; Liu, Pei Feng; Ger, Luo Ping; Tsai, Kuo Wang^*

来源：Anticancer Research, 2017, 37(3): 1059-1066.

DOI：10.21873/anticanres.11417

• microRNA
• miR-21
• next-generation sequencing
• oral cancer

Dysfunctional microRNAs (miRNAs) play a crucial role in oral squamous cell carcinoma (OSCC) progression. In the present study, we performed next-generation sequencing for miRNA profiling of the OSCC tissues and corresponding adjacent normal tissues in two patients with OSCC. We observed that 45 miRNAs were substantially up-regulated and 17 miRNAs were down-regulated in OSCC tissues. Since information on the biological role of miR-21-3p (passenger strand) in OSCC is limited, the expression levels of miR-21-3p were further evaluated in 95 OSCC tissue samples by a stem-loop real-time polymerase chain reaction. Our results revealed that miR-21-3p is significantly overexpressed in the OSCC tissues compared with the corresponding adjacent normal tissues ( p< 0.001). High miR-21-3p expression levels were significantly correlated with N classification (p= 0.042). After transfection with a miR-21-3p inhibitor (antagomir), the invasive ability of the OSCC cells was significantly abrogated. Altogether, our findings indicated that miR-21-3p plays a crucial oncogenic role in cell metastasis during OSCC progression.

• 出版日期2017-3
• 单位中山大学