科研之友
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摘要
The sequence-related amplified polymorphism (SRAP) technique was applied to identify the genetic difference between the Whole Red (WR) population and the Whole White (WW) population. The specific SRAP band, namely SR2,7173 bp (SR indicates the first two letters of SRAP) from WR and WW, was identified from the amplified bands of 12 primer pairs (PP) screened from 88 SRAP PP. After gel extraction, cloning and sequencing of specific band from the two populations, the sequence was submitted to database of Genome Sequence Survey. blast analysis showed that this SRAP fragment shared high similarity to functional genes of Danio rerio. Four primers (2226 bases) were designed according to the sequence information. Then, polymerase chain reaction amplification was carried out in the two populations. The experimental results also showed that among four sequence-characterized amplified region (SCAR) markers, only SC-3 (154 bp) developing from SR2,7173 bp (me2-em7) showed specificity to WR. Examination with a large sample size showed that SCAR 3 (SC-3) could be positively amplified with 85% frequency in the WR population rather than in the WW population. The results indicated that the SC-3 marker could be used as a specific molecular marker for population identification, providing an effective, easy and rapid method for discriminating different populations and conducting genetic analysis.
