Brain derived neurotrophic factor release from layer-by-layer coated agarose nerve guidance scaffolds

作者：Lynam Daniel A; Shahriari Dena; Wolf Kayla J; Angart Phillip A; Koffler Jacob; Tuszynski Mark H; Chan Christina; Walton Patrick; Sakamoto Jeffrey^*

来源：Acta Biomaterialia, 2015, 18: 128-131.

DOI：10.1016/j.actbio.2015.02.014

• Agarose
• Hydrogel
• BDNF
• Nerve
• Controlled release

Agarose nerve guidance scaffolds (NGS) seeded with cells expressing brain derived neurotrophic factor (BDNF) have demonstrated robust nerve regeneration in the rat central nervous system. The purpose of this work was to explore whether agarose NGS coated with hydrogen-bonded layer-by-layer (HLbL) could provide an acellular method of delivering prolonged and consistent dosages of active BDNF. Our results show that HLbL-coated agarose NGS could release BDNF over 10 days in consistent dosages averaging 80.5 +/- 12.5 (SD) ng/mL. Moreover, the BDNP released from HLbL was confirmed active by in vitro cell proliferation assays. To our knowledge, this is the first report demonstrating that HLbL assembled onto a hydrogel can provide consistent, prolonged release of active BDNF in clinically relevant dosages.

• 出版日期2015-5