A mixture of isothiocyanates induces cyclin B1- and p53-mediated cell-cycle arrest and apoptosis of human T lymphoblastoid cells

作者：Fimognari C^*; Nusse M; Berti F; Lori R; Cantelli Forti G; Hrelia P

来源：Mutation Research-Fundamental and Molecular Mechanisms of Mutagenesis, 2004, 554(1-2): 205-214.

DOI：10.1016/j.mrfmmm.2004.04.009

• isothiocyanates
• cell cycle
• apoptosis
• cyclin B1
• p53
• bax

As with other candidate chemopreventive agents, most of our knowledge on the biological effects of isothiocyanates (the many sulfur-containing metabolites found in cruciferous vegetables) comes from studies of single natural or synthetic compounds. To investigate whether the biological/chemopreventive effects of administration of single isothiocyanates can differ from those of a mixture of isothiocyanates, we tested the effects of a mixture of four different isothiocyanates on cell-cycle progression and apoptosis in human T leukemia Jurkat cells, and identified some of the molecular pathways triggered by the mixture. The mixture affected critical points of the cell cycle via modulation of the expression of cyclin B L Moreover, it induced apoptosis. mediated by an increase in p53 and bax (expression of bcl-2 was unaffected). Comparison of the data with those previously obtained with the single isothiocyanates under identical experimental conditions provides evidence that the quantitative effects of a single, specific isothiocyanate can be significantly different from those of an isothiocyanate mixture at realistic doses.

• 出版日期2004-10-4