Diet Composition Modulates Expression of Sirtuins and Renin-Angiotensin System Components in Adipose Tissue

作者：de Pinho Lucineia; Oliveira Andrade Joao Marcus; Paraiso Alanna; Maia Filho Aristides Batista; Feltenberger John D; Sena Guimaraes Andre Luiz; Batista de Paula Alfredo Mauricio; Caldeira Antonio Prates; de Carvalho Botelho Ana Cristina; Campagnole Santos Maria Jose; Sousa Santos Sergio Henrique

来源：Obesity, 2013, 21(9): 1830-1835.

DOI：10.1002/oby.20305

Objective: The aim of this study was to evaluate the expression of RAS components and SIRTs enzymes in the adipose tissue of mice fed diets with different macronutrient composition. %26lt;br%26gt;Design and Methods: The body weight, food intake, and energy intake (kcal) were evaluated. Blood parameters (insulin sensitivity, glucose tolerance, total cholesterol, HDL-C triglyceride, and glucose levels) were also assessed. Real-time PCR was performed in epididymal adipose tissue samples to analyze the expression of renin, angiotensinogen (AGT), angiotensin-converting enzyme 1 and 2 (ACE and ACE2), and SIRTs 1-7. Male FVB/N mice were divided into 5 groups (N = 10 each) that were fed with experimental diets for 60 days. Test diets were divided into standard (ST), AIN-93M, high glucose (HG), high protein (HP) and high lipid (HL). %26lt;br%26gt;Results: The main results showed that HL diet treatment induced reduction in HDL-C and triglyceride plasma levels; increased ACE (Ang II marker) expression and decreased ACE2 (Ang-[1-7] catalyzer) expression in adipose tissue; and also increased SIRT4 expression. %26lt;br%26gt;Conclusion: Diets with high lipid content induced a degenerative state associated with deregulation of adipose tissue enzymes expression.

• 出版日期2013-9